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Standard Capillary Sequencing:

 

 

 

 

 

 

 

 

 

 

 

Description

Macrogen Inc. offers DNA sequencing using state-of-the-art robotics and instrumentation to generate high quality sequence data at affordable prices. We have successfully sequenced countless samples of PCR products, plasmids, and BACs from customers all over the world, and our DNA sequencing services are highly rated for their turnaround time, quality of results, and competitive prices.

 

Features
   ● 3730xl DNA analyzer
   ● Real time monitoring from registration to result delivery using LIMS        (Laboratory Information Management System)
   ● Result delivery within 24 hours.
   ● Performing fundamental analysis service at free of charge      

      (Investigating homology for the protein and DNA sequence level  

      through BlastN or BlastX) 

Sample preparation
  Template
      ● Plasmid : Concentration 100ng/㎕, Volume 20㎕
      ● PCR product (purified) : Concentration 50ng/㎕, Volume 20㎕
      ● PCR product (non-purified) : Concentration 100ng/㎕, Vol 20~30㎕
         (Example) In case of 300bp of PCR product, if you send 30ng/㎕ of a

          sample then better results are obtained(100bp≒10ng/ ㎕). It is

          recommended to check the PCR product concentration through

          electrophoresis rather than Nano-drop.
  Primer
      ● Concentration : 5 pmole/㎕ (5pM), Volume : 20㎕
         (Example) For 5 Reactions: 20㎕, as the reaction number increases

          increment 2㎕ each.
      ● Please separate template and primer when you send primers.
      ● Primer Tm : 55~60℃, GC content : 40%~60%.

 

Results
     ● Based on normal plasmid template basis, guarantee phred score

        more than 20, read-length more than 650 bp 
 

 

 

 

 

 

 

 

 

Custom DNA Sequencing
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Difficult DNA Templates Sequencing

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Primer Walking Service 

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16/18s (ITS 5.8s) rRNA Sequencing

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