Sanger Sequencing

Capillary Electrophoresis Sequencing (CES) is a service that analyzes DNA sequences using a biochemical method. The CES service of Macrogen maximizes customer satisfaction with various service lineups such as identification services, which involves the differentiation and identification of species of living organisms, fragment analysis services, and customized sequencing services newly applied from the existing sequencing method, as well as standard sequencing that is the most common service. CES is used in a wide range of fields such as a basic research in molecular biology, breeding research, genetic disease research, paternity confirmation and forensic research, and has become a key in the continued development of life sciences.Aside from general sequencing services, Macrogen’s CES service provides additional customized services (gDNA extraction, primer synthesis, PCR amplification, and purification) according to customer requests. Separate online order system is also available for customers’ convenience so that they can request a desired service. Monitoring of all processes and follow-up for the results are provided for each ordered sample.
Standard Sequencing

sequences PCR products and plasmid DNA requested by customers. Results released within 24-48 hours.

Identification Service

16S/18S/26S rRNA and ITS region full sequencing is a service that analyzes sequences by amplifying ribosomal RNA genes of bacteria and fungi (filamentous fungus and yeast) and confirming homology of target microorganisms using an rRNA database (NCBI).

Primer Walking

generally used to read long sequences (2 to 10kb) that cannot be read at once by single primer extension. End sequencing is performed with a primer specified by the customer, and the internal primer is designed and produced based on the results. This is extended continuously by redesigning the internal primer in a suitable location from the results obtained by the new primer’s reaction with the same template.

Fragment Analysis Service

separates and analyzes amplified PCR products according to fragments using a primer marked by a fluorescent label. Service types include:

-Microsatellite instability
-Amplified fragment length polymorphism (AFLP) analysis
-Terminal restriction fragment length polymorphism (T-RFLP) analysis
-
Relative fluorescent quantization
-
Sequence-related amplified polymorphism

PCR Optimization

a customized service that performs and analyzes the entire process from primer design to PCR amplification, sequencing and BI report when the customer provides references together with cell/gDNA in relation to the desired target region.

NGS Validation Service

verifies data using Sanger sequencing to improve the accuracy and reliability of data after using the NGS service

MLST Analysis Service

classify strains with the same species such as bacteria and fungi by analyzing the correlation of each ST (Sequence Type). Genetic/molecular evolutionary relations can be verified by the combination of alleles for each strain in the same species.

PYrosequencing

using pyrophosphate (PPi) generated when the polymerization of nucleotide with DNA occurs.

Cloning Service

performed by separating a specific gene or DNA section and duplicating it in large amounts. . The service is provided by inserting a PCR product in the vector (T-vector or blunt-vector) provided by Macrogen or via the vector from the customer (sub-cloning).

Next-Generation Sequencing

NGS (Next Generation Sequencing) is the method of reading a genome by dividing it into many pieces, assembling the obtained sequence pieces and analyzing the sequence of the whole genome. There has been rapid progress in technologies related to NGS since its commercialization in year 2000, which has produced a large volume of genome information for use in research. Especially as the cost and time for genome analysis gradually decreased, demand for genome analysis for living organisms including humans increased rapidly. Recently clinical studies based on NGS are actively conducted, mostly in the medical field, and many studies are in progress to identify genetic variance and the functions of new genes. This will quicken the realization of precision medicine that can provide treatment optimized for each person based on the patient’s genome. 

  Macrogen produces a large amount of genome information every year using various kinds of equipment, provides quicker and more accurate genome analysis service at an economical price to help studies conducted by researchers, and offers a diagnostic service to the public. We provide genome analysis services suitable for research, such as whole genome, exome, transcriptome, epigenome and metagenome, and bioinformatics results based on the data. Offering a total genomic solution to researchers all over the world based on our extensive experiences and know-how accumulated over the years, Macrogen leads the field of precision medicine utilizing NGS technology.
Whole Genome Sequencing
reading the entire genome and analyzing related genetic information.
Exome Sequencing
selective analysis in an exon area known to be where genes exist. It is a more effective and economical way of performing research than whole genome resequencing because it selectively analyzes only the genes obtained from current studies.
Targeted Sequencing
selective analysis on the areas researchers are interested in.
Transcriptome Sequencing

determine how gene expression changes under different conditions.

Metagenome Sequencing

identify which microbial communities are present in a sample collected in a certain environment, and to identify their interactions and roles.

Epigenome Sequencing

confirms that the function of a certain gene is expressed by external environmental factors.