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New England Biolabs

NEB EpiMark N6-Methyladenosine Enrichment Kit (Cat No. E1610)

NEB EpiMark N6-Methyladenosine Enrichment Kit (Cat No. E1610)

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The Epimark® N6-Methyladenosine Enrichment Kit can be used to enrich m6A modified RNA in immunoprecipitation protocols for downstream analysis by next-generation RNA sequencing or RT-qPCR.

  • Complete protocol for enrichment of m6A-modified –RNA and analysis by RT- qPCR included
  • RNA controls (m6A modified and unmodified RNA) enable monitoring of enrichment and depletion
  • Antibody supplied in a ready-to-use solution form
Catalog # Concentration Size
E1610S Not Applicable 20 reactions

 

 

The EpiMark N6-Methyladenosine Enrichment Kit contains a rabbit monoclonal antibody specific for N6-Methyladenosine (m6A). The kit also contains two control RNAs, one with m6A modification (Gaussia luciferase) and one without (Cypridina luciferase) to monitor enrichment and depletion. The GLuc RNA control was transcribed in the presence of 20% m6ATP and 80% ATP.
This kit can be used to enrich m6A modified RNA in immunoprecipitation protocols for downstream analysis by next-generation RNA sequencing or RT-qPCR. Modified RNA is isolated from a fragmented RNA sample by binding to the N6-Methyladenosine antibody attached to Protein G Magnetic Beads. After multiple wash and clean-up steps, the enriched RNA is eluted in nuclease-free water and is ready for further analysis.

Epimark N6-Methyladenosine Enrichment Kit Workflow
Figure 1. Workflow

Each kit contains sufficient reagents to perform 10 x 2-round immunoprecipitations with starting amounts of up to 250 µg of ribosome depleted or poly A+ purified RNA. It contains sufficient reagents for 5 x 2-round immunoprecipitations when using up to 250 µg of total RNA.

  • Protocols, Manuals & Usage

    • Manuals

      The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Product Name: NEB EpiMark N6-Methyladenosine Enrichment Kit (Cat No. E1610)

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